Laboratory of Mammalian Cell Design and Engineering
Laboratory of Mammalian Cell Design and Engineering
Laboratory of Mammalian Cell Design and Engineering
Laboratory of Mammalian Cell Design and Engineering
Laboratory of Mammalian Cell Design and Engineering
GRADUATE STUDENTS
All publications
‡corresponding author, *equal contribution
Preprints
1. Kim SH, Shin S, Baek M, Xiong K, Karottki KJC, Hefzi H, Grav LM, Pedersen LE, Kildegaard HF, Lewis NE, Lee JS‡, Lee GM‡. Identification of hyperosmotic stress-responsive genes in Chinese hamster ovary cells via genome-wide virus-free CRISPR/Cas9 screening. Genome-wide, virus-free CRISPR-Cas9 screen identifies hyperosmotic stress-responsive genes in Chinese hamster ovary cells. bioRxiv. (2023)
2023
46. Kim SM, Lee J, Lee JS. Implementation of Ubiquitous Chromatin Opening Elements as Artificial Integration Sites for CRISPR/Cas9-Mediated Knock-In in Mammalian Cells. Eng. Life Sci., 23(4), e2200047 (2023)
45. Kim D, Lee JS. Limitations of the Plasmid-Based Cas9-Zinc Finger Fusion System for Homology-Directed Knock-In in Chinese Hamster Ovary Cells. Biotechnol. Bioproc. E., 10.1007/s12257-022-0348-6 (2023)
2022
44. Min H, Kim SM, Kim D, Lee S, Lee S, Lee JS. Hybrid cell line development system utilizing site-specific integration and methotrexate-mediated gene amplification in Chinese hamster ovary cells. Front. Bioeng. Biotechnol., 10, 977193 (2022)
43. Shin S, Kim SH, Park J, Lee JS‡, Lee GM‡. Recombinase-mediated cassette exchange-based screening of a CRISPR/Cas9 library for enhanced recombinant protein production in human embryonic kidney cells: Improving resistance to hyperosmotic stress. Metab. Eng., 72, 247-258 (2022)
42. Kyeong M, Lee JS. Endogenous BiP reporter system for simultaneous identification of ER stress and antibody production in Chinese hamster ovary cells. Metab. Eng., 72, 35-45 (2022)
41. Kim SH, Baek M, Park S, Shin S, Lee JS‡, Lee GM‡. Improving the secretory capacity of CHO producer cells: The effect of controlled Blimp1 expression, a master transcription factor for plasma cells. Metab. Eng., 69, 73-86 (2022)
2021
40. Shin SW*, Kyeong M*, Lee JS. Next-Generation Cell Engineering Platform for Improving Recombinant Protein Production in Mammalian Cells. In: Pörtner R. (eds) Cell Culture Engineering and Technology. Cell Engineering, vol 10. Springer, Cham. https://doi.org/10.1007/978-3-030-79871-0_7 (2022)
39. Jang MH*, Min H*, Lee JS. Enhancement of Transgene Expression by Mild Hypothermia is Promoter-Dependent in HEK293 Cells. Life, 11(9), 901 (2021)
38. Xiong K, la Cour Karottki KJ, Hefzi H, Li S, Grav LM, Li S, Spahn P, Lee JS, Ventina I, Lee GM, Lewis NE, Pedersen LE. An optimized genome-wide, virus-free CRISPR screen for mammalian cells. Cell Rep. Methods, 1, 100062 (2021)
37. Shin S, Kim SH, Lee JS‡, Lee GM‡. Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins. ACS Synth. Biol., 10(7), 1715-1727 (2021)
36. Karottki KJC, Hefzi H, Li S, Pedersen LE, Spahn PN, Joshi C, Ruckerbauer D, Bort JAH, Thomas A, Lee JS, Borth N, Lee GM, Kildegaard HF‡, Lewis NE‡. A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes. Metab. Eng., 66, 114-122 (2021)
35. Shin SW*, Kim D*, Lee JS. Controlling Ratios of Plasmid-Based Double Cut Donor and CRISPR/Cas9 Components to Enhance Targeted Integration of Transgenes in Chinese Hamster Ovary Cells. Int. J. Mol. Sci., 22(5), 2407 (2021)
34. Kwak JM*, Lee Y*, Shin SW*, Lee JS. Hydroxyurea selection for enhancing homology-directed targeted integration of transgenes in CHO cells. New Biotechnol., 62, 26-31 (2021)
2020
33. Shin SW, Lee JS. CHO Cell Line Development and Engineering via Site-specific integration: Challenges and Opportunities. Biotechnol. Bioproc. E., 25, 633-645 (2020)
32. Lee Y, Kwak JM, Lee JS. Endogenous p21-Dependent Transgene Control for CHO Cell Engineering. ACS Synth. Biol., 9(7), 1572-1580 (2020)
31. Shin S, Kim SH, Shin SW, Grav LM, Pedersen LE, Lee JS‡, Lee GM‡. Comprehensive Analysis of Genomic Safe Harbors as a Target Site for Stable Expression of Heterologous Gene in HEK293 Cells. ACS Synth. Biol., 9(6), 1263-1269 (2020)
30. Shin SW, Lee JS. Optimized CRISPR/Cas9 Strategy for Homology-Directed Multiple Targeted Integration of Transgenes in CHO Cells. Biotechnol. Bioeng., 117(6), 1895-1903 (2020)
29. Karottki KJC, Hefzi H, Xiong K, Shamie I, Hansen AH, Li S, Pedersen LE, Li S, Lee JS, Lee GM, Kildegaard HF‡, Lewis NE‡. Awakening dormant glycosyltransferases in CHO cells with CRISPRa. Biotechnol. Bioeng., 117(2), 593-598 (2020)
2019
28. Ha TK*, Lee JS*, Lee GM. Platform Technology for Therapeutic Protein Production. Cell Culture Engineering: Recombinant Protein Production, 1-22 (2019) https://doi.org/10.1002/9783527811410.ch1
27. Sergeeva D, la Cour Karottki KJ, Lee JS, Kildegaard HF. CRISPR Toolbox for Mammalian Cell Engineering. Cell Culture Engineering: Recombinant Protein Production, 185-206 (2019) https://doi.org/10.1002/9783527811410.ch8
26. Sergeeva D, Camacho-Zaragoza JM, Lee JS, Kildegaard HF. CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells. Methods Mol. Biol., 1961, 213-232 (2019)
25. Xiong K*, Marquart KF*, la Cour Karottki KJ*, Li S, Shamie I, Lee JS, Gerling S, Yeo NC, Chavez A, Lee GM, Lewis NE‡, Kildegaard HF‡. Reduced Apoptosis in Chinese Hamster Ovary Cells via Optimized CRISPR Interference. Biotechnol. Bioeng., 116(7), 1813-1819 (2019)
24. Lee JS‡, Kildegaard HF, Lewis NE, Lee GM‡. Mitigating Clonal Variation in Recombinant Mammalian Cell Lines. Trends Biotechnol., 37(9), 931-942 (2019)
2018
23. Lee JS‡, Park JH, Ha TK, Samoudi M, Lewis NE, Palsson BO, Kildegaard HF, Lee GM‡. Revealing key determinants of clonal variation in transgene expression in recombinant CHO cells using targeted genome editing. ACS Synth. Biol., 7(12), 2867-2878 (2018) DOI: 10.1021/acssynbio.8b00290
22. Grav LM, Sergeeva D, Lee JS, Marin de Mas I, Lewis NE, Andersen MR, Nielsen LK, Lee GM, Kildegaard HF. Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation. ACS Synth. Biol., 7(9), 2148-2159 (2018) DOI: 10.1021/acssynbio.8b00140
21. Petersen SD, Zhang J, Lee JS, Jakočiūnas T, Grav LM, Kildegaard HF, Keasling JD, Jensen MK. Modular 5'-UTR hexamers for context-independent tuning of protein expression in eukaryotes. Nucleic Acids Res., 46(21), e127 (2018)
20. Baek E, Lee JS, Lee GM. Untangling the mechanism of 3-methyladenine (3-MA) in enhancing the specific productivity: Transcriptome analysis of recombinant CHO cells treated with 3-MA. Biotechnol. Bioeng., 115(9), 2243-22543 (2018)
2017
19. Grav LM, la Cour Karottki KJ, Lee JS, Kildegaard HF. Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells. Methods Mol. Biol., 1603, 101-118 (2017)
2016
18. Hefzi H*, Ang KS*, Hanscho M*, Bordbar A, Ruckerbauer D, Lakshmanan M, Orellana CA, Baycin-Hizal D, Huang Y, Ley D, Martinez VS, Kyriakopoulos S, Jiménez NE, Zielinski DC, Quek LE, Wulff T, Arnsdorf J, Li S, Lee JS, Paglia G, Loira N, Spahn PN, Pedersen LE, Gutierrez JM, King ZA, Lund AM, Nagarajan H, Thomas A, Abdel-Haleem AM, Zanghellini J, Kildegaard HF, Voldborg BG, Gerdtzen ZP, Betenbaugh MJ, Palsson BO, Andersen MR, Nielsen LK, Borth N‡, Lee DY‡, Lewis NE‡. A Consensus Genome-scale Reconstruction of Chinese Hamster Ovary Cell Metabolism. Cell Syst., 3(5), 434–443 (2016)
- Press releases at phys.org, UCSD Health Sciences
17. Lee JS, Grav LM, Pedersen LE, Lee GM, Kildegaard HF. Accelerated Homology-Directed Targeted Integration of Transgenes in Chinese Hamster Ovary Cells via CRISPR/Cas9 and Fluorescent Enrichment. Biotechnol. Bioeng., 113(11), 2518-2523 (2016)
- Most Accessed in 2016 in this journal; Outstanding Best Poster Award, Cell Culture Engineering XV, CA, USA
16. Baek E, Kim CL, Kim MG, Lee JS, Lee GM. Chemical Inhibition of Autophagy: Examining its Potential to Increase the Specific Productivity of Recombinant CHO Cell Lines. Biotechnol. Bioeng., 113(9), 1953-1961 (2016)
2015
15. Lee JS, Grav LM, Lewis NE, Kildegaard HF. CRISPR/Cas9 genome engineering of CHO cell factories: Application and perspectives. Biotechnol. J., 10(7), 979-994 (2015)
- Most Cited/Accessed in 2015-2017 in this journal
14. Grav LM*, Lee JS*, Gerling S, Kallehauge TB, Hansen AH, Kol S, Lee GM, Pedersen LE, Kildegaard HF. One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment. Biotechnol. J., 10(9), 1446-1456 (2015)
- Most Cited/Accessed in 2015, 2016 in this journal
13. Lee JS, Kallehauge TB, Pedersen LE, Kildegaard HF. Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway. Sci. Rep., 5, 8572 (2015)
2013
12. Kim YJ, Baek E, Lee JS, Lee GM. Autophagy and its implication in Chinese hamster ovary cell culture. Biotechnol. Lett., 35(11), 1753-1763 (2013)
11. Lee JS, Ha TK, Park JH, Lee GM. Anti-Cell Death Engineering of CHO Cells: Co-Overexpression of Bcl-2 for Apoptosis Inhibition and Beclin-1 for Autophagy Induction. Biotechnol. Bioeng., 110(8), 2195-2207 (2013)
2012
10. Sathyamurthy M, Lee JS, Kim YJ, Park JH, Jeong JY, Jang JW, Lee GM. Overexpression of PACEsol improves BMP-7 processing in recombinant CHO cells. J. Biotechnol., 164(2), 336-339 (2012)
9. Lee JS, Lee GM. Estimation of autophagy pathway genes for autophagy induction: Controlled overexpression of Atg9A does not induce autophagy in recombinant Chinese hamster ovary cells. Biochem. Eng. J., 68, 221-226 (2012)
8. Lee JS, Ha TK, Lee SJ, Lee GM. Current state and perspectives on erythropoietin production. Appl. Microbiol. Biotechnol., 95(6), 1405-1416 (2012)
7. Lee JS, Lee GM. Rapamycin Treatment Inhibits CHO Cell Death in a Serum-Free Suspension Culture by Autophagy Induction.
Biotechnol. Bioeng., 109(12), 3093-3102 (2012)
6. Lee JS, Kim YJ, Kim CL, Lee GM. Differential induction of autophagy in Caspase-3/7 downregulating and Bcl-2 overexpressing rCHO cells upon NaBu treatment. J. Biotechnol., 161(1), 34-41 (2012)
5. Lee JS, Lee GM. Effect of Sodium Butyrate on Autophagy and Apoptosis in Chinese Hamster Ovary Cells. Biotechnol. Prog., 28(2), 349-357 (2012)
4. Lee JS, Lee GM. Monitoring of autophagy in Chinese hamster ovary cells using flow cytometry. Methods, 56(3), 375-382 (2012)
2011
3. Han YK, Ha TK, Lee SJ, Lee JS, Lee GM. Autophagy and Apoptosis of Recombinant Chinese Hamster Ovary Cells during Fed-Batch Culture: Effect of Nutrient Supplementation. Biotechnol. Bioeng., 108(9), 2182-2192 (2011)
2010
2. Lee JS, Park HJ, Kim YH, Lee GM. Protein reference mapping of dihydrofolate reductase-deficient CHO DG44 cell lines using 2-dimensional electrophoresis. Proteomics, 10(12), 2292-2302 (2010)
2007
1. Sung YH*, Lee JS*, Park SH, Koo J, Lee GM. Influence of co-down-regulation of caspase-3 and caspase-7 by sodium butyrate-induced apoptotic cell death of Chinese hamster ovary cells producing thrombopoietin. Metab. Eng., 9(5-6), 452-464 (2007)